Nanotopography Promotes Pancreatic Differentiation of Human Embryonic Stem (ES) Cells and Induced Pluripotent Stem (iPS) Cells
SCIE
SCOPUS
- Title
- Nanotopography Promotes Pancreatic Differentiation of Human Embryonic Stem (ES) Cells and Induced Pluripotent Stem (iPS) Cells
- Authors
- Kim, JH; Hyung Woo Kim; Kyoung Je Cha; Jiyou Han; Yu Jin Jang; Kim, DS; Kim, JH
- Date Issued
- 2016-03
- Publisher
- AMER CHEMICAL SOC
- Abstract
- Although previous studies suggest that nano topographical features influence properties and behaviors of stem cells, only a few studies have attempted to derive clinically useful somatic cells from human pluripotent stem cells using nanopatterned surfaces. In the present study, we report that polystyrene nanopore-patterned surfaces significantly promote the pancreatic differentiation of human embryonic and induced pluripotent stem cells. We compared different diameters of nanopores and showed that 200 nm nanopore-patterned surfaces highly upregulated the expression of PDX1, a critical transcription factor for pancreatic development, leading to an approximately 3-fold increase in the percentage of differentiating PDX1(+) pancreatic progenitors compared with control flat surfaces. Furthermore, in the presence of biochemical factors, 200 nm nanopore-patterned surfaces profoundly enhanced the derivation of pancreatic endocrine cells producing insulin, glucagon, or somatostatin. We also demonstrate that nanopore-patterned surface-induced upregulation of PDX1 is associated with downregulation of TAZ, suggesting the potential role of TAZ in nanopore-patterned surface-mediated mechano-transduction. Our study suggests that appropriate cytokine treatments combined with nanotopographical stimulation could be a powerful tool for deriving a high purity of desired cells from human pluripotent stem cells.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/36453
- DOI
- 10.1021/ACSNANO.5B06985
- ISSN
- 1936-0851
- Article Type
- Article
- Citation
- ACS NANO, vol. 10, no. 3, page. 3342 - 3355, 2016-03
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- There are no files associated with this item.
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