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| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Li, Qian | - |
| dc.contributor.author | Chang, Young-Tae | - |
| dc.date.accessioned | 2018-06-15T05:15:33Z | - |
| dc.date.available | 2018-06-15T05:15:33Z | - |
| dc.date.created | 2017-09-08 | - |
| dc.date.issued | 2006 | - |
| dc.identifier.issn | 1754-2189 | - |
| dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/50273 | - |
| dc.description.abstract | This protocol outlines a methodology for the preparation and characterization of three RNA-specific fluorescent probes (E36, E144 and F22) and their use in live cell imaging. It describes a detailed procedure for their chemical synthesis and purification; serial product characterization and quality control tests, including measurements of their fluorescence properties in solution, measurement of RNA specificity and analysis of cellular toxicity; and live cell staining and counterstaining with Hoechst or DAPI. Preparation and application of these RNA imaging probes takes 1 week. | - |
| dc.language | English | - |
| dc.publisher | NATURE PUBLISHING GROUP | - |
| dc.relation.isPartOf | NATURE PROTOCOLS | - |
| dc.subject | LIVING CELLS | - |
| dc.subject | FLUORESCENCE | - |
| dc.title | A protocol for preparing, characterizing and using three RNA-specific, live cell imaging probes: E36, E144 and F22 | - |
| dc.type | Article | - |
| dc.identifier.doi | 10.1038/nprot.2006.484 | - |
| dc.type.rims | ART | - |
| dc.identifier.bibliographicCitation | NATURE PROTOCOLS, v.1, no.6, pp.2922 - 2932 | - |
| dc.identifier.wosid | 000251155700051 | - |
| dc.date.tcdate | 2019-02-01 | - |
| dc.citation.endPage | 2932 | - |
| dc.citation.number | 6 | - |
| dc.citation.startPage | 2922 | - |
| dc.citation.title | NATURE PROTOCOLS | - |
| dc.citation.volume | 1 | - |
| dc.contributor.affiliatedAuthor | Chang, Young-Tae | - |
| dc.identifier.scopusid | 2-s2.0-34548852559 | - |
| dc.description.journalClass | 1 | - |
| dc.description.journalClass | 1 | - |
| dc.description.wostc | 25 | - |
| dc.type.docType | Article | - |
| dc.subject.keywordPlus | LIVING CELLS | - |
| dc.subject.keywordPlus | FLUORESCENCE | - |
| dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
| dc.description.journalRegisteredClass | scie | - |
| dc.description.journalRegisteredClass | scopus | - |
| dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
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Related Researcher
- 장영태CHANG, YOUNG TAE
- Dept of Chemistry