A protocol for preparing, characterizing and using three RNA-specific, live cell imaging probes: E36, E144 and F22
SCIE
SCOPUS
- Title
- A protocol for preparing, characterizing and using three RNA-specific, live cell imaging probes: E36, E144 and F22
- Authors
- Li, Qian; Chang, Young-Tae
- Date Issued
- 2006
- Publisher
- NATURE PUBLISHING GROUP
- Abstract
- This protocol outlines a methodology for the preparation and characterization of three RNA-specific fluorescent probes (E36, E144 and F22) and their use in live cell imaging. It describes a detailed procedure for their chemical synthesis and purification; serial product characterization and quality control tests, including measurements of their fluorescence properties in solution, measurement of RNA specificity and analysis of cellular toxicity; and live cell staining and counterstaining with Hoechst or DAPI. Preparation and application of these RNA imaging probes takes 1 week.
- Keywords
- LIVING CELLS; FLUORESCENCE
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/50273
- DOI
- 10.1038/nprot.2006.484
- ISSN
- 1754-2189
- Article Type
- Article
- Citation
- NATURE PROTOCOLS, vol. 1, no. 6, page. 2922 - 2932, 2006
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- There are no files associated with this item.
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