Self-Assembled Nanoconstructs Modified with Amplified Aptamers Inhibited Tumor Growth and Retinal Vascular Hyperpermeability via Vascular Endothelial Growth Factor Capturing
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- Title
- Self-Assembled Nanoconstructs Modified with Amplified Aptamers Inhibited Tumor Growth and Retinal Vascular Hyperpermeability via Vascular Endothelial Growth Factor Capturing
- Authors
- Lee, J.; Lee, B.J.; Lee, Y.M.; Park, H.; Kim, J.H.; Kim, W.J.
- Date Issued
- 2017-05
- Publisher
- AMER CHEMICAL SOC
- Abstract
- Here, nanoconstructs consisting of a DNA-amplified aptamer with a biocompatible polymer backbone for capturing target biomolecules are presented. First, the polymer-DNA nanoconstructs were prepared by hybridization of two complementary single-stranded DNAs that were each conjugated to a dextran polymer backbone. The designed polymer-DNA amplified aptamer nanoconstructs (PA-aNCs) were then prepared by utilizing polymer-DNA nanoconstructs conjugated with an aptamer (PA-NCs) using a rolling circle amplification reaction to amplify the aptamer. These PA-aNCs were successfully applied to alleviate tumor growth and vascular endothelial growth factor (VEGF)-induced retinal vascular hyperpermeability in vivo through the highly effective capture of human VEGF as a target molecule. These PA-aNCs could be used as therapeutic agent for anti-VEGF therapy by efficiently capturing human VEGF. ? 2017 American Chemical Society.
- Keywords
- aptamer; complementary DNA; dextran; DNA; polymer; single stranded DNA; vasculotropin; animal experiment; animal model; Article; biocompatibility; blood vessel permeability; conjugation; controlled study; DNA hybridization; gene amplification; growth inhibition; human; human cell; mouse; nonhuman; priority journal; retina blood vessel; synthesis; tumor growth
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/92108
- DOI
- 10.1021/acs.molpharmaceut.6b00949
- ISSN
- 1543-8384
- Article Type
- Article
- Citation
- MOLECULAR PHARMACEUTICS, vol. 14, no. 5, page. 1460 - 1468, 2017-05
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