DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sugahara, M. | - |
dc.contributor.author | Nakane, T. | - |
dc.contributor.author | Masuda, T. | - |
dc.contributor.author | Suzuki, M. | - |
dc.contributor.author | Inoue, S. | - |
dc.contributor.author | Song, C. | - |
dc.contributor.author | Tanaka, R. | - |
dc.contributor.author | Nakatsu, T. | - |
dc.contributor.author | Mizohata, E. | - |
dc.contributor.author | Yumoto, F. | - |
dc.contributor.author | Tono, K. | - |
dc.contributor.author | Joti, Y. | - |
dc.contributor.author | Kameshima, T. | - |
dc.contributor.author | Hatsui, T. | - |
dc.contributor.author | Yabashi, M. | - |
dc.contributor.author | Nureki, O. | - |
dc.contributor.author | Numata, K. | - |
dc.contributor.author | Nango, E. | - |
dc.contributor.author | Iwata, S. | - |
dc.date.accessioned | 2018-07-17T10:46:47Z | - |
dc.date.available | 2018-07-17T10:46:47Z | - |
dc.date.created | 2017-12-21 | - |
dc.date.issued | 2017-04 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/92118 | - |
dc.description.abstract | Serial femtosecond crystallography (SFX) allows structures of proteins to be determined at room temperature with minimal radiation damage. A highly viscous matrix acts as a crystal carrier for serial sample loading at a low flow rate that enables the determination of the structure, while requiring consumption of less than 1 mg of the sample. However, a reliable and versatile carrier matrix for a wide variety of protein samples is still elusive. Here we introduce a hydroxyethyl cellulose-matrix carrier, to determine the structure of three proteins. The de novo structure determination of proteinase K from single-wavelength anomalous diffraction (SAD) by utilizing the anomalous signal of the praseodymium atom was demonstrated using 3,000 diffraction images. ? 2017 The Author(s). | - |
dc.language | English | - |
dc.publisher | Nature Publishing Group | - |
dc.relation.isPartOf | Scientific Reports | - |
dc.title | Hydroxyethyl cellulose matrix applied to serial crystallography | - |
dc.type | Article | - |
dc.identifier.doi | 10.1038/s41598-017-00761-0 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Scientific Reports, v.7, no.1 | - |
dc.identifier.wosid | 000398545900022 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.number | 1 | - |
dc.citation.title | Scientific Reports | - |
dc.citation.volume | 7 | - |
dc.contributor.affiliatedAuthor | Song, C. | - |
dc.identifier.scopusid | 2-s2.0-85018432559 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 3 | - |
dc.description.isOpenAccess | Y | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | FREE-ELECTRON LASER | - |
dc.subject.keywordPlus | X-RAY-DIFFRACTION | - |
dc.subject.keywordPlus | PROTEIN-STRUCTURE DETERMINATION | - |
dc.subject.keywordPlus | CUBIC PHASE INJECTOR | - |
dc.subject.keywordPlus | FEMTOSECOND CRYSTALLOGRAPHY | - |
dc.subject.keywordPlus | STRUCTURAL-CHANGES | - |
dc.subject.keywordPlus | SYNCHROTRON-RADIATION | - |
dc.subject.keywordPlus | RESOLUTION STRUCTURE | - |
dc.subject.keywordPlus | CRYSTAL-STRUCTURE | - |
dc.subject.keywordPlus | TEMPERATURE | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
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