Hydroxyethyl cellulose matrix applied to serial crystallography
SCIE
SCOPUS
- Title
- Hydroxyethyl cellulose matrix applied to serial crystallography
- Authors
- Sugahara, M.; Nakane, T.; Masuda, T.; Suzuki, M.; Inoue, S.; Song, C.; Tanaka, R.; Nakatsu, T.; Mizohata, E.; Yumoto, F.; Tono, K.; Joti, Y.; Kameshima, T.; Hatsui, T.; Yabashi, M.; Nureki, O.; Numata, K.; Nango, E.; Iwata, S.
- Date Issued
- 2017-04
- Publisher
- Nature Publishing Group
- Abstract
- Serial femtosecond crystallography (SFX) allows structures of proteins to be determined at room temperature with minimal radiation damage. A highly viscous matrix acts as a crystal carrier for serial sample loading at a low flow rate that enables the determination of the structure, while requiring consumption of less than 1 mg of the sample. However, a reliable and versatile carrier matrix for a wide variety of protein samples is still elusive. Here we introduce a hydroxyethyl cellulose-matrix carrier, to determine the structure of three proteins. The de novo structure determination of proteinase K from single-wavelength anomalous diffraction (SAD) by utilizing the anomalous signal of the praseodymium atom was demonstrated using 3,000 diffraction images. ? 2017 The Author(s).
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/92118
- DOI
- 10.1038/s41598-017-00761-0
- ISSN
- 2045-2322
- Article Type
- Article
- Citation
- Scientific Reports, vol. 7, no. 1, 2017-04
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