T cell microvilli constitute immunological synaptosomes that carry messages to antigen-presenting cells
SCIE
SCOPUS
- Title
- T cell microvilli constitute immunological synaptosomes that carry messages to antigen-presenting cells
- Authors
- Kim, Hye-Ran; Mun, YeVin; Lee, Kyung-Sik; Park, Yoo-Jin; Park, Jeong-Su; Park, Jin-Hwa; Jeon, Bu-Nam; Kim, Chang-Hyun; Jun, Youngsoo; Hyun, Young-Min; Kim, Minsoo; Lee, Sang-Myeong; Park, Chul-Seung; Im, Sin-Hyeog; Jun, Chang-Duk
- Date Issued
- 2018-09
- Publisher
- NATURE PUBLISHING GROUP
- Abstract
- Microvilli on T cells have been proposed to survey surfaces of antigen-presenting cells (APC) or facilitate adhesion under flow; however, whether they serve essential functions during T cell activation remains unclear. Here we show that antigen-specific T cells deposit membrane particles derived from microvilli onto the surface of cognate antigen-bearing APCs. Microvilli carry T cell receptors (TCR) at all stages of T cell activation and are released as large TCR-enriched, T cell microvilli particles (TMP) in a process of trogocytosis. These microvilli exclusively contain protein arrestin-domain-containing protein 1, which is directly involved in membrane budding and, in combination with vacuolar protein-sorting-associated protein 4, transforms large TMPs into smaller, exosome-sized TMPs. Notably, TMPs from CD4(+) T cells are enriched with LFA-2/CD2 and various cytokines involved in activating dendritic cells. Collectively, these results demonstrate that T cell microvilli constitute "immunological synaptosomes" that carry T cell messages to APCs.
- Keywords
- CD2 antigen; cell membrane protein; retina S antigen; T lymphocyte receptor; unclassified drug; vacuolar protein sorting associated protein 4; lymphocyte antigen receptor; antigen; cell component; immune system; membrane; protein; animal cell; antigen presenting cell; Article; CD4+ T lymphocyte; cell budding; cell membrane; cell size; cell surface; cell transformation; dendritic cell; exosome; human; human cell; microvillus; mouse; nonhuman; phenomena and functions of biological membrane; protein expression; synaptosome; T lymphocyte; T lymphocyte activation; trogocytosis; animal; antigen presenting cell; HEK293 cell line; Jurkat cell line; membrane microparticle; metabolism; microvillus; physiology; synaptosome; ultrastructure; Animals; Antigen-Presenting Cells; CD4-Positive T-Lymphocytes; Cell-Derived Microparticles; Dendritic Cells; HEK293 Cells; Humans; Jurkat Cells; Mice; Microvilli; Receptors, Antigen, T-Cell; Synaptosomes
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/94628
- DOI
- 10.1038/s41467-018-06090-8
- ISSN
- 2041-1723
- Article Type
- Article
- Citation
- NATURE COMMUNICATIONS, vol. 9, no. 1, 2018-09
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- There are no files associated with this item.
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