탈세포화 췌장 유래 바이오 잉크 개발 및 췌도세포 배양 시스템에서의 응용

Abstract

The transplantation of pancreatic islets is promising treatment for patients of type 1 diabetes, suffering hypoglycaemia and secondary complications. The primary goal of this treatment is to supply sufficient amount of insulin in the bloodstream, however, several limitations still remain. After transplantation, most of the islets in the intrahepatic transplant site have difficult condition to survive due to low engraftment rate and hostile environment. In case of subcutaneous transplantation, the viability and function of the implanted islet is diminished because of the low vascularity occurring hypoxic condition. With the convergence of 3D bioprinting, bioink and stem cell biology approaches, tissue engineering offers a promising alternative. Pancreatic islets were encapsulated in decellularized ECM based bioink for providing islets with a physiologically relevant microenvironment, which could improve the viability and functionality of the cells. We also optimized co-culture condition using vascular cells that can provide beneficial microenvironmental cues. Finally, we developed a subcutaneously implantable pancreatic construct via 3D cell printing technique. This construct can regulate blood glucose level in diabetic animal models and has shown a significant potential of the advanced islet delivery method for the treatment of type 1 diabetes.