On-chip biosynthesis of GM1 pentasaccharide-related complex glycans
SCIE
SCOPUS
- Title
- On-chip biosynthesis of GM1 pentasaccharide-related complex glycans
- Authors
- Kim, Chang Sup; Heo, Hye Ryoung; Seo, Jeong Hyun; Cha, Hyung Joon
- Date Issued
- 2019-01
- Publisher
- ROYAL SOC CHEMISTRY
- Abstract
- A functional glycan chip combined with on-chip enzymatic glycosylation was developed to prepare complex glycan sources and to apply glycan-involved applications simultaneously. GM3 trisaccharide, GM2 tetrasaccharide, and GM1 pentasaccharide were successfully directly biosynthesized on lactose-immobilized surfaces through three consecutive glycosyltransferase reactions along with small amounts of enzymes and donors, without any additional processes. Biosynthesized GM1 pentasaccharide-related complex glycans were demonstrated to provide information on the substrate specificity of whole cholera toxin. Thus, the proposed on-chip glycan biosynthesis system can provide a new direction toward obtaining complex glycan sources and complex glycan-involved applications such as glycan-protein interaction analysis and glycan biomarker-based diagnosis.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/95329
- DOI
- 10.1039/c8cc06526h
- ISSN
- 1359-7345
- Article Type
- Article
- Citation
- CHEMICAL COMMUNICATIONS, vol. 55, no. 1, page. 71 - 74, 2019-01
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- There are no files associated with this item.
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